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. 2016 Oct 10;11(10):e0164523. doi: 10.1371/journal.pone.0164523

Fig 10. Effect of δ-toxin knockout on the colony-spreading activity in SA564 and FRP3757 strains.

Fig 10

A. The colony-spreading activities of the hld-wild-type strain (WT) or the δ-toxin knockout strains (Δhld1, Δhld2) of SA564 or FRP3757 strains were examined. S. aureus overnight cultures were spotted on 0.24% soft agar plates and incubated at 37°C (SA564 and FRP3757) or 30°C (FRP3757). The photographs were obtained at 6 h incubation (SA564), 7 h incubation (FRP3757, 37°C), and 28 h incubation (FRP3757, 30°C). B. The diameters of the giant colony in A were measured. Data are the means±standard errors of two independent experiments performed in triplicate. Asterisks indicate Student’s t-test p-value between WT and Δhld1 or between WT and Δhld2 less than 0.05.