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. 2016 Oct 10;11(10):e0163845. doi: 10.1371/journal.pone.0163845

Fig 8. VGCC activation and mycobacterial infection synergistically lead to suppression of pro-inflammatory cytokine responses in mouse macrophages.

Fig 8

Bone marrow derived mouse macrophages (Panel A&B) either were infected with 2 MOI of M. bovis BCG or stimulated with 50nM of BAYK8644 or both for 24 h. For Panel A, culture supernatant was processed for the estimation of indicated cytokines and bars represent the amount of cytokine in pg/ml. For Panel B, cells were processed for measuring surface densities of indicated cytokine receptors by FACS and bars represent MFI of indicated groups of three independent experiments (n = 3). The results were analyzed by one way ANOVA followed by Tukey’s post hoc multiple comparison test. The star above the bar represents the P value between unstimulated/uninfected or control and the corresponding group of that bar in each panel. ns = P>0.05; * = P< 0.05; ** = P<0.01; *** = P<0.001 and **** = P<0.0001.