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. 2016 Oct 10;11(10):e0164463. doi: 10.1371/journal.pone.0164463

Fig 6. Effect of stabilisation of the forward primer on the performance of the panFMDV-3D RT-qPCR assay.

Fig 6

FMDV isolate O/TUR/2/92 was tested with the panFMDV-3D RT-qPCR assay using different primer combinations. Non-linear regression models were fitted to the raw fluorescence data of each replicate and the resulting models were amalgamated into a single replicate model using the replist function from the qpcR package [38] (S4 File). The figure shows the replicate model of each primer combination with error bars representing 1 standard deviation. (nt: non-tailed, t: tailed, zna: zipped nucleic acid).