Table 1. Effect of primer tailing on Cq values of the panFMDV-3D RT-qPCR assay.

Viral genomic RNA was tested with the panFMDV-3D RT-qPCR assay using either non-tailed or tailed primers. Five isolates of the FMDV reference panel (n = 50) showed markedly lower Cq values when both primers were tailed. The table lists the difference (average ± standard deviation) between reactions containing non-tailed or tailed primers of the 5 aberrant isolates.

isolate	non-tailed–tailed (Cq value)
Asia 1/CAM/2/91	4.51 ± 0.12
C/PHI/11/89	3.79 ± 0.05
O/PHI/7/75	3.98 ± 0.11
O/TUR/2/92	3.04 ± 0.23
SAT2/ZIM/19/89	1.83 ± 0.04