Figure 5. Disruption of the complex formation of Cbx7-PRC1 facilitates the targeting of Cbx7 to chromatin.

(A) Normalized histograms of the log maximum likelihood diffusion coefficient ${\displaystyle D_m}$ for HaloTag-Cbx7 in wild-type mES cells replicated from Figure 1E and for HaloTag-Cbx7 in Ring1a^−/−/Ring1b^−/− (N = 29 cells, n = 2600 trajectories) and Bmi1^−/−/Mel18^−/− (N = 27 cells, n = 6859 trajectories) mES cells. The histograms were fitted with a three-component Gaussian. (B) Fraction of the CB (red), ID (cyan), and FD (green) population for HaloTag-Cbx7 in wild-type mES cells replicated from Figure 1F and for HaloTag-Cbx7 in Ring1a^−/−/Ring1b^−/− and Bmi1^−/−/Mel18^−/− mES cells. Results are means ± SD. (C–D) Residence time (C) and fraction (D) of the stable chromatin-bound population for HaloTag-Cbx7 in wild-type mES cells replicated from Figure 4C and D and for HaloTag-Cbx7 in Ring1a^−/−/Ring1b^−/− (N = 18 cells, n = 4849 trajectories) and Bmi1^−/−/Mel18^−/− (N = 27 cells, n = 3484 trajectories) mES cells. (E) Survival probability for HaloTag-Cbx7 in wild-type mES cells replicated from Figure 4E, and for HaloTag-Cbx7 in Ring1a^−/−/Ring1b^−/− and Bmi1^−/−/Mel18^−/− mES cells.

DOI: http://dx.doi.org/10.7554/eLife.17667.045

Figure 5—figure supplement 1. Cumulative frequency distribution of the dwell times for determining the residence times of HaloTag-Cbx7 in Ring1a^−/−/Ring1b^−/− and Bmi1^−/−/Mel18^−/− mES cells.

Cumulative frequency distribution of the dwell times for HaloTag-Cbx7 replicated from Figure 4B and for HaloTag-Cbx7 in Ring1a^−/−/Ring1b^−/− (N = 38 cells, n = 7825 trajectories) and Bmi1^−/−/Mel18^−/− (N = 47 cells, n = 8142 trajectories) mES cells. The cumulative frequency distributions were fitted with a two-component exponential decay model.

DOI: http://dx.doi.org/10.7554/eLife.17667.047