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. Author manuscript; available in PMC: 2017 Oct 1.

Published in final edited form as: J Allergy Clin Immunol. 2016 Jun 14;138(4):1089–1097.e3. doi: 10.1016/j.jaci.2016.04.042

After separation of blood using Ficoll-Hypaque density centrifugation, eosinophils were enriched using magnetic affinity column purification. A. Transcript levels of hPGDS were quantified using PCR with sybr-green detection. Data (mean±SEM) reflect relative expression of each gene in comparison to the housekeeping gene EF1α (ΔCT). Control samples (n=13) are depicted in black triangles, Asthma (n=12) in blue squares and AERD (n=14) in red circles. B. Measurement of hPGDS protein by Western hybridization. Eosinophils from three control, asthmatic and AERD subjects were collected and electrophoresed on a denaturing polyacrylamide gel and probed with rabbit antibody directed against hPGDS and ß-actin as a loading control. C. Semi-quantitative analysis of hPGDS protein levels. (AERD n=9, Asthma n=7, and Control n=10) *p<0.02 as compared to Control.

After separation of blood using Ficoll-Hypaque density centrifugation, eosinophils were enriched using magnetic affinity column purification. A. Transcript levels of hPGDS were quantified using PCR with sybr-green detection. Data (mean±SEM) reflect relative expression of each gene in comparison to the housekeeping gene EF1α (ΔCT). Control samples (n=13) are depicted in black triangles, Asthma (n=12) in blue squares and AERD (n=14) in red circles. B. Measurement of hPGDS protein by Western hybridization. Eosinophils from three control, asthmatic and AERD subjects were collected and electrophoresed on a denaturing polyacrylamide gel and probed with rabbit antibody directed against hPGDS and ß-actin as a loading control. C. Semi-quantitative analysis of hPGDS protein levels. (AERD n=9, Asthma n=7, and Control n=10) *p<0.02 as compared to Control.