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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Mol Immunol. 2016 Aug 30;78:38–47. doi: 10.1016/j.molimm.2016.08.016

Figure 3.

Figure 3

Complement regulators Crry, CD55 and CD59 change in placenta and kidney cortex in RUPP compared to Sham animals. A. mRNA was isolated from placenta and kidney cortex and Delta-delta Ct method of relative quantification was used to determine fold change in mRNA expression compared to actin with the change in Sham animals defined as 1. Values represent the mean ± SE of fold change obtained from 4–7 animals for placenta and 7–9 animals for kidney. *p<0.05 vs Sham. B. Immunohistochemistry was conducted as described in Materials and Methods, and staining graded by a blinded observer from 0–3, negative to strongly positive. Representative images at 200X magnification are provided. Values for staining intensity represent the mean ± SE of scores obtained from placenta from 4–5 animals. *p<0.05 vs Sham