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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: J Virol Methods. 2016 Aug 31;237:159–165. doi: 10.1016/j.jviromet.2016.08.023

Table 1.

Primers used for ddPCR and qPCR of Pan/99wt-His virus templates

Gene Segment Location Forward Primer Reverse Primer
PB2 Terminal1 50 150
PB2 Internal2 834 932
PB1 Terminal 50 150
PB1 Internal 749 845
PA Terminal 50 150
PA Internal 857 935
HA Terminal 51 150
HA Internal 745 901
NP Terminal 49 149
NP Internal 668 761
NA Terminal 50 152
NA Internal 683 846
M Terminal 50 151
M Internal 392 502
NS Terminal 69 194
NS Internal 359 497
1

Terminal primers are located within the first (3’) 150 nt and designed to detect both defective interfering particles and standard segments.

2

Internal primers are beyond 359 nt and designed detect only standard segments.