. 2016 Jul 14;68(10):797–806. doi: 10.1007/s00251-016-0934-x

Table 3.

Codon sites predicted to be under positive selection in the three Japanese Rana species, referred to as positively selected sites

Species	Codon sites predicted to be under positive selection
	α1 domain																		α2 domain																					α3 domain
	5	9	11	22	24	31	34	45	62	63	66	67	69	70	73	74	77	81	94	95	97	99	114	116	117	124	125	133	135	145	149	154	156	160	164	167	171	175	177	255	258
R. japonica	˄	˄	˅		˅		˅	˄		˄	˄				˅	˄		˅	˄							˄			˄				˄	˄		˄		˄
R. ornativentris	˅	X	X	˄	˄	˅	X			X	X	˅		X	X	X	˄	X		X	X	˅	˅	X			˅		˅	˅		˄	X	X	˅	˄			˄	˅
R. t. tagoi	˄	X	X		˅				˄	˅	˄	X	˄	X	X			X	˄	˄	˅	˄	˅		X			˄			˄	X	X	˄		˅	˄			˄	X
Human^a	Y	Y		Y	Y		Y	Y		Y	Y	Y	Y	Y	Y	Y	Y	Y		Y	Y	Y	Y	Y						Y	Y	Y		Y	Y	Y	Y	Y		–	–
Other frogs^b	–	Y	Y		Y		Y	Y	Y	Y	Y	Y	Y	Y	Y			Y	Y	Y	Y	Y	Y	Y	Y				Y	Y		Y	Y	Y		Y		Y			Y

Sites are considered to be under positive selection if detected by at least two (˄), three (˅) or all (X) of the four methods used (omegaMap, FEL, REL and MEME). Many PSS were also deduced or predicted to be selected sites (Y) in humans or other frogs. Codon sites are based on the alignment in Fig. 1. ‘–’ indicates the site was not tested

^aDeduced PBR sites from HLA based on Bjorkman et al (1987) and Saper et al (1991)

^bPredicted selected sites in other frog species based on Kiemnec-Tyburczy et al (2012) and Zhao et al (2013)