Table 2.

RT-qPCR amplification data of five developmentally important genes in Echinococcus granulosus sensu stricto (G1) and the reference genes

Gene name (GeneDB prediction)	GeneDB model no.	Primer: forward/reverse Amplicon size/qPCR Tm
Eg-fz4 Frizzled (FZD.1)	EgrG_000636500	5′-TCGGTCGCTACAAACGGG/ 5′-GCTGACTGATTCAACGCCC 206 bp/58 °C
Eg-fgfrl FGF receptor-like (FGFR)	EgrG_000842900	5′-TCCTGCCGTCAGTACTCC/ 5′-GCGTGGATAACTGTTCTCC 216 bp/58 °C
eg-rcd1 Cell diferentiation protein (rcd1)	EgrG_000966200	5′-TGCCATACCTATGAGCGATTC/ 5′-CATCTACTAGCTGCGCTGC 208 bp/58 °C
Eg-smadD Co-smad (Smad4)	EgrG_000217400	5′-GGGCACACATTCTGCTCC/ 5′-TCCATTGTTGGTGCCGCC 215 bp/58 °C
eg-braf B-Raf (Raf)	EgrG_001079900	5′-AAGGCGTTCAAGAATGAGGT/ 5′-GTGTAGGTAATCCATGCCC 218 bp/58 °C
eg-tbp TATA-box binding protein (tbp)	EgrG_000972300	5′-TTCCAGCGCTCAGGCACACA/ 5′-CGTGCGCTTTGAGCTATCCGTCT 165 bp/58 °C
eg-ef1α Elongation factor 1α (Ef1 α)	EgrG_000982200	5′-TTTGAGAAAGAGGCGGCTGAGATG/ 5′-TAATAAAGTCACGATGACCGGGCG 174 bp/58 °C
eg-cyp Cyclophilin (Cyclophilin)	EgrG_000920600	5′-CGACATCTCCATTGGCGGTAAGC/ 5′-TTGTATCCGAAACCCTTCTCACCG 120 bp/58 °C