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. 2016 Sep 30;6(9):e478. doi: 10.1038/bcj.2016.87

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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FAXDC2 expedites megakaryopoiesis in murine bone marrow cells. (a) c-kit-positive progenitor cells isolated from primary murine bone marrow cells were transduced with control lentiviral vector (Ctrl) or FAXDC2-overexpressing (FAXDC2) vector to undergo megakaryocytic differentiation with thrombopoietin (TPO) for 6 days. The resultant cells were stained with phycoerythrin (PE)-CY7-conjugated anti-CD41 antibody and analyzed by flow cytometry (left panel). Bar graph (right panel) was the statistics of left panel. (b) The resultant cells were stained with PE-conjugated anti CD42 antibody and analyzed by flow cytometry (left panel). Bar graph (right panel) was the statistics of left panel. (c) The resultant cells were stained with anti-CD41-PE-CY7 and DAPI. The CD41⁺cells were gated for analysis of DNA content. The DNA content that is >4N is represented (left panel). Bar graph (right panel) was the statistics of left panel. (d) The control (Ctrl) or FAXDC2-overexpressing (FAXDC2) cells were harvested for Wright-Giemsa stains. The stained cells were photographed under microscopy at the bright view of the microscope (magnification × 20 or magnification × 40). Scale Bar: 100 μm. (e) The control (Ctrl) or FAXDC2-overexpressing (FAXDC2) cells were harvested for quantitative real-time PCR (RT-PCR) at mRNA level. The expression of RUNX1 was measured. *P<0.05 compared with control.