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. 2016 Sep 27;5(9):e367. doi: 10.1038/mtna.2016.74

Figure 1.

Figure 1

A schematic illustration of the EcR-based singular transgene inducible switch. The depicted Gal4-VP16-EcR chimeric transcriptional activator is constitutively produced under the control of a CMV promoter. The chimeric proteins remain in the cytosol in the absence of an ecdysone agonist, tebufenozide. Administration of tebufenozide triggers the translocation of Gal4-VP16-EcR from the cytosol to the nucleus, where it binds to 10×UAS and stimulates basal transcriptional machinery to initiate transcription of the downstream transgene. The entire transgene regulatable unit was transferred en bloc into a nonviral vector or an adenoviral vector. Abbreviations: Teb, tebufenozide; CMV, cytomegalovirus; EcR, ecdysone receptor; UAS, upstream activation sequence; 10×UAS, 10 tandem repeats of UAS.