. 2016 Sep 12;9(9):964–969. doi: 10.14202/vetworld.2016.964-969

Table-1.

Details of CPE on the MDCK cells at different passage levels.

Passage level	CPV isolate no. 2

	24 h p.i	48 h p.i	72 h p.i	96 h p.i	120 h p.i
1^st	-	-	-	-	-
2^nd	-	-	-	-	-
3^rd	-	-	-	-	-
4^th	-	-	-	-	-
5^th	-	-	-	-	+
6^th	-	-	+	+	+
7^th	-	+	+	++	+++

-: No visible change in cell monolayer, +: Slight rounding, ++: Rounding and slight sloughing/detachment of cell monolayer, +++: Complete sloughing/detachment of cell monolayer, CPE: Cytopathic effects, MDCK: Madin-Darby canine kidney, p.i.: Post infection