Table 2. Kinetic constants of TyrBm on its natural substrates and HQ.
Substrate | Km (mM) | Vmax (μmole min−1 mg−1) | kcat (s−1) | kcat/Km (s−1 mM−1) |
---|---|---|---|---|
L-tyrosine | 0.082 ± 0.006 | 3.62 ± 0.06 | 2.1 | 25.6 |
L-dopa | 0.24 ± 0.02 | 30.3 ± 0.6 | 17.8 | 74.2 |
HQ | 0.27 ± 0.05 | 19 ± 1 | 11.3 | 41.9 |
Data was extracted from Figs 1, 2 and Supplementary Fig. S1. Each value represents the mean ± SD of five independent experiments.