Figure 5. Distribution of FtsZ in the PEG/DNA LLPS system.
(a) Representative confocal images of FtsZ within the PEG/DNA (3:1) emulsions in the absence and presence of 1 mM GTP. Total FtsZ concentration was 12 (GDP) and 8 μM (GTP). A schematic illustration of the disposition of FtsZ within the phases is depicted on the right. (b) Concentration dependence of the distribution of FtsZ-GDP within the mixture as determined by fluorescence together with an illustration on the right. Data are the average of at least 3 independent measurements ± SD.