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. 2016 Oct 11;6:34865. doi: 10.1038/srep34865

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Dorsal skin of Ptch1^+/−/SKH-1 mice was treated either with vehicle or PAO (100 μg/mouse) for different time intervals (0–16 h) and assessed for ROS production and cell death markers. (A) Fluorescence-based microphotographs showing ROS production at various time (0–16 h) intervals as assessed by staining with CM-H2DCFDA probe. (B-I) Western blot analysis of cleaved caspase-3. Histogram showing dentiometric analysis of western blots. (B-II) Microphotographs analysis of fluorescent-based green TUNEL positive cells observed at various time (0–16 h) points. TUNEL positive cells were observed as early as within 4 h following exposure with PAO to mouse skin. Please note that apoptotic cells at this time were only found in hair follicles. However, at later time-points inter-follicular epidermis was also positive. Insets represent magnified area of the TUNEL positive cells.