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. 2016 Oct 11;6:35061. doi: 10.1038/srep35061

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Sections 2 μm in thickness were prepared for hematoxylin-eosin staining, and 4 μm sections were prepared for Klüver-Barrera staining. Scale bars: 500 μm. (a) A hematoxylin-eosin section of cerebrum. The anterior commissure and corpus callosum appear as eosin-stained (pink) structures. (b) A Klüver-Barrera section of cerebrum. Myelinated axonal tracts in the anterior commissure, corpus callosum, and striatum appear as blue structures. (c) A hematoxylin-eosin section of cerebellum. Granular layers appear as hematoxylin-stained (purple) structures.