Figure 8. Sensitivity of FgChs single and double deletion mutants to cell wall stress agents.

(a) Serial dilutions of conidial suspension of each strain were spotted on MM (CK) and MM supplemented with 0.2 g/l congo red (CR). All the plates were incubated at 25 °C for 3 days. (b) Mycelial plugs of PH-1, ΔFgChs1, ΔFgChs2 and ΔFgChs2/1 were inoculated on MM (CK) and MM supplemented with 0.2 g/l CR. All the plates were incubated at 25 °C for 3 and 5 days. (c) After treatment with cellulase, lysozyme and snailase at 30 °C for 30 min, mycelia of the mutants ΔFgChs1, ΔFgChs2, ΔFgChs7, ΔFgChs5 and all the double deletion mutants were well digested and released abundant protoplasts. Bar = 10 μm.