DHM attenuated MPTP-induced GSK-3β activation in both the striatum (A) and SNc (B). Male C57BL/6 mice were divided into four groups: saline, 25 mg/kg MPTP, 10 mg/kg DHM and 10 mg/kg DHM+25 mg/kg MPTP. For the groups with DHM treatment, mice were given 10 mg/kg DHM (ip) daily for a total of 13 d (3 d before the start of MPTP, during MPTP administration (7 d) and 3 d after the end of MPTP). The striatum and SNc were dissected and processed for Western blotting, as described in Methods. Mean±SD. n=3. *P<0.05 vs control. ##P<0.01 vs MPTP group.