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. 2016 Jun 6;37(10):1381–1390. doi: 10.1038/aps.2016.61

Figure 3.

Figure 3

LGH00168 induced necroptosis in A549 cells. (A) PI/HO staining indicated the induction of cellular swelling and plasma membrane rupture. (B) The Annexin V/PI assay indicated the induction of necrosis. (C) The SSC/FSC parameters showed cell death of LGH00168-treated cells. (D) AO/EB staining indicated the induction of severe LMP. (E) JC-1 staining indicated the collapse of MMP in LGH00168-treated cells. (F) A caspase 8 activity assay indicated the inhibition of caspase 8 activity. (G) The RIP1 inhibitor Nec-1 partially reversed LGH00168-induced PMP. (H) The RIP1 inhibitor Nec-1 partially reversed LGH00168-induced necroptosis. *P<0.05, **P<0.01.