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. 2016 Aug 29;37(10):1370–1380. doi: 10.1038/aps.2016.62

Figure 3.

Figure 3

Dex treatment leads to decreased mineralized matrix of skull in AB strain larval zebrafish. (A) Images of skull dorsal aspect of alizarin red stained AB strain larval zebrafish at 9 dpf exposed to 0, 5, 10, 15, and 20 μmol/L Dex for 6 d; (B) The influence of Dex on skull mineralization area analyzed by IPP6.0 in 9 dpf AB strain larval zebrafish; (C) The influence of Dex on skull mineralization IOD analyzed by IPP6.0 in 9 dpf AB strain larval zebrafish. Notes: IPP6.0 (Image-Pro Plus image analysis software version 6.0); IOD (integral optical density); Veh (vehicle control, 0.1% dimethyl sulphoxide); Dex5 (Dex 5 μmol/L); Dex10 (Dex 10 μmol/L); Dex15 (Dex 15 μmol/L); Dex20 (Dex 20 μmol/L). Data are given as mean±SD. n≥9. *P<0.05 vs vehicle control.