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. 2016 Aug 29;37(10):1370–1380. doi: 10.1038/aps.2016.62

Figure 5.

Figure 5

Salvianolic acid B increases skull mineralized matrix in intact AB strain larval zebrafish. (A) Images of skull dorsal aspect of alizarin red stained AB strain larval zebrafish at 9 dpf treated with 0, 0.2, 0.5, 1, and 2 μmol/L Sal B for 6 d; (B) the influence of Sal B on skull mineralization area analyzed by IPP6.0 in 9-dpf AB strain larval zebrafish and (C) the influence of Sal B on skull mineralization IOD analyzed by IPP6.0 in 9-dpf AB strain larval zebrafish. Notes: IPP6.0 (Image-Pro Plus image analysis software version 6.0); IOD (integral optical density); Con (control, egg water); S0.2 (Sal B 0.2 μmol/L); S0.5 (Sal B 0.5 μmol/L); S1 (Sal B 1 μmol/L); S2 (Sal B 2 μmol/L). Data are given as mean±SD. n≥9. *P<0.05 vs vehicle control.