Table 1.

Performance of RQPCR by consensus germline or entirely patient-specific primers/probes

Cases	Consensus germline primers/probes				Patient-specific primers/probes
	Diagnostic marrow DNA as standards				Diagnostic marrow DNA as standards				CDR3-cloned plasmid as standards
	Sensitivity	QR	R 2	Slope	Sensitivity	QR	R 2	Slope	Sensitivity	QR	R 2	Slope
Dx53	N/A	10−2	N/A	N/A	10−5	10−4	0.996	−3.30	10−5	10−4	0.992	−3.44
M3	ND	ND	ND	ND	5 × 10−4	5 × 10−4	0.989	−3.48	10−4	10−4	0.997	−3.54
M8	10−4	5 × 10−4	0.985	−3.38	10−4	10−3	0.999	−3.46	10−4	5 × 10−4	0.999	−3.61
M4	ND	ND	ND	ND	10−4	10−3	0.984	−3.60	10−5	10−3	0.999	−3.54
Dx31	ND	ND	ND	ND	10−4	5 × 10−4	0.998	−3.45	10−4	10−4	0.998	−3.47
M5	10−4	10−4	0.995	−3.41	5 × 10−4	5 × 10−4	0.995	−3.67	10−5	10−4	0.998	−3.51
M10	N/A	5 × 10−4	N/A	N/A	N/A	5 × 10−4	N/A	N/A	10−4	10−4	0.994	−3.51
Dx49	5 × 10−4	10−3	0.982	−3.37	10−4	10−3	0.986	−3.26	10−4	5 × 10−4	0.999	−3.76
M1	ND	ND	ND	ND	5 × 10−4	10−3	0.986	−3.48	10−4	5 × 10−4	0.998	−3.52
M6	ND	ND	ND	ND	N/A	5 × 10−4	N/A	N/A	10−5	5 × 10−4	0.997	−3.72
M2	N/A	N/A	N/A	N/A	ND	ND	ND	ND	ND	ND	ND	ND
M7	N/A	10−2	N/A	N/A	N/A	10−2	N/A	N/A	10−4	10−3	0.999	−3.56
M9	N/A	10−2	N/A	N/A	N/A	10−2	N/A	N/A	10−4	10−4	0.997	−3.55

Abbreviations: QR quantitative range, R ² correlation coefficient, N/A not applicable, ND not done due to absence of target band in qualitative PCR or no need (for M2 in patient-specific approach)