Table 2.

Mismatches against consensus germline reverse primers and/or probes

Cases	Mismatches against primers			Mismatches against probes
	Primers	Primer binding site sequences	No.	Probes	Probe binding site sequences	No.
Dx53	R-JH6-intron	gcagaGaGGaaGggccctagagt	4	T-JH6	cacggtcaccgtctcctcaggCaagaa	1
M3	R-JH4-intron	cagagAAaaTATCACagagaggttgt	8	T-JH1.2.4.5	ccctggtcaccgtctcctcaggtg	0
M8	R-JH4-intron	cagagCtaaagcaggagagaggttgt	1	T-JH1.2.4.5	ccctggtcaccgtctcctcaggtg	0
M4	R-JH4-intron	cagGAttTaagTaggGgagaCgttgt	6	T-JH1.2.4.5	ccctggtcaccgtctcctcaggtg	0
Dx31	R-JH4-intron	TagaCCCaaagTaggagagaCAttAt	8	T-JH1.2.4.5	Tcctggtcaccgtctcctcaggtg	1
M5	R-JH3-intron	aggcagaaggaaagccatcttac	0	T-JH3	cCagggacaatggtcaccgtctcttca	1
M10	R-JH6-intron	gcagaaaacaaaggccctagagt	0	T-JH6	cacggtcaccgtctcctcaggtaagaa	0
Dx49	R-JH6-intron	–	?	T-JH6	cacggtcaccgtctcctcaggtaagaT	1
M1	R-JH4-intron	–	?	T-JH1.2.4.5	cTGt---	≥2
M6	R-JH3-intron	–	?	T-JH3	caagggacaat---	?
M2	R-JH4-intron	cagagttaaagcaggagagaggttgt	0	T-JH1.2.4.5	ccctggtcGccgtctcctcaggtg	1
M7	R-JH5-intron	agagagggggtggtgaggact	0	T-JH1.2.4.5	ccctggtcaccgtctcctcaggtg	0
M9	R-Jk1	gatcacttcatagacacagggaacag	0	T-Jk1	tggaaatcaaacgtgag	0

Uppercase letters in sequences indicate mismatches. “?” indicates mismatches cannot be identified based on available sequencing data