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. 2016 Oct 10;215(1):77–93. doi: 10.1083/jcb.201603019

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© 2016 Redli et al.

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Figure 4. — The MT-binding capability of the Ska complex is required for Aurora B activity. Immunofluorescence images (A, C, E, G, and I) and quantification (B, D, F, H, and J) of relative Hec1-pS44, KNL1-pS24, MCAK, histone H3-pS10, and AurB-pT232 intensities, respectively, in HeLa cells stably expressing GFP-tagged and siRNA-resistant wild-type Ska1 (WT) or the MT-binding deficient mutants Ska1^{R155A, R236A, R245A} (ARG) and Ska1^1-131 (ΔMTBD), respectively, treated for 48 h with control or Ska1 siRNAs (n = 25, n = 40–55, n = 17–26, n = 35–40, and n = 81–105 cells per condition, respectively, from one to four experiments). Note that spindle association of GFP-Ska1^WT is not visible in all cells because of the simultaneous cell permeabilization and fixation. Horizontal lines depict mean. Asterisks show statistical significance (Student’s t test, unpaired). ****, P ≤ 0.0001; ***, P ≤ 0.001. Bars, 5 µm.