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. 2015 Oct 23;138(6):1516–1527. doi: 10.1002/ijc.29881

Figure 1.

Figure 1

Effect of CTX and ZOL on colon cancer cell lines. (a) The expression levels of EGFR, p‐EGFR and RAS were determined via western blot analysis in six colorectal cancer cell lines. β‐actin was used as the loading control. (b) The in vitro sensitivity of six colorectal cancer cells to CTX was determined using the MTT assay. These cells were treated with 0.001–1000 nM CTX alone for 72 hr. (c) Synergistic effect of CTX and ZOL. SW48 and LS174T cells were treated with 1–100 nM CTX and/or 0–163.36 μM ZOL for 72 hr. (d) SW48 and LS174T cells were treated with ZOL (100 μM) for 96–120 hr. Cells were subjected to analysis of caspase‐3 by western blot. (e) The long‐term effects of combination treatment with CTX and ZOL. Representative plates are shown. Numbers of colonies are indicated. Error bars in (b), (c) and (e) indicate standard deviation (SD).