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. 2015 Oct 6;14(3):1046–1054. doi: 10.1111/pbi.12477

Figure 2.

Figure 2

Characterization of transgenic plants. (a) Sterile tassel from ms45 plant. (b) Fertile tassel from transgenic ms45 plant containing pPG 47::Bt1:zm‐aa1// pM s5126::Ms45//p35S::PAT . (c–i) Pollen from transgenic plant containing pPG 47::Bt1:zm‐aa1//35SEN‐ pU bi::PAT::DsRed‐Express. (c and d) Mature pollen stained with fluorescein diacetate (c) and also shown under colour filter for red fluorescent proteins (d). (e) Mature pollen sample in (c) stained with potassium iodide. (f–i) In vivo pollen germination. (f and g) DsRed‐Express pollen without pollen tube and non‐DsRed‐Express pollen with pollen tube shown under visible light (f) and under colour filter for DsRed‐Express (g). (h and i) Abnormal pollen tube growth from a DsRed‐Express pollen grain shown under visible light (h) and under colour filter for DsRed‐Express (i). (j) Pollen tube growth from a control transgenic plant containing pU bi::YFP . Arrow in f, and h–j shows pollen tubes. (k–n) Characterization of seed DsRed2 expression and seed sorting. (k) Ear harvested from a transgenic plant bearing pM s5126::Ms45// pPG 47::Bt1:zm–aa1//35SEN‐ pLTP 2::DsRed2(Alt1). (l and m) Non‐DsRed2 and DsRed2 seeds shown under green filter (l) and under red filter (m). (n) Seeds shown on seed sorting machine as they flow from top to bottom. Arrows in k–n show DsRed2 seeds. Bar = 100 μm.