Table 2.

Male fertility of self‐pollinated progenies from transgenic SPT maintainer plants

Number of plants with observed phenotype/total number of plants (%)
Promoter‐gene combination and transformant	Fully fertile	Partially fertile	Completely sterile
Fluorescent seeds
pPG47::Bt1:zm‐aa1//pMs5126::Ms45//35SEN‐pLTP2::DsRed2(Alt1) Event E6499.105.6.7	28/28 (0)	0/28 (0)	0/28 (0)
pMs5126::Ms45//pPG47::Bt1:zm‐aa1//35SEN‐pLTP2::DsRed2(Alt1) Event DP‐32138‐1	188/194 (96.9)	6/194 (3.1)	0/194 (0)
pMs45::Ms45//pPG47::Bt1:zm‐aa1//35SEN‐pLTP2::DsRed2(Alt1) Event E6611.22.8.2	19/21 (90.5)	2/21 (9.5)	0/21 (0)
pPG47::Bt1:zm‐aa1//pMs45::Ms45//pLTP2::DsRed2(Alt1) Event E6499.75.6.3	31/37 (83.8)	6/37 (16.2)	0/37 (0)
Nonfluorescent seeds
Nontransgenic progeny of event E6499.105.6.7	0/14 (0)	0/14 (0)	14/14 (100)
Nontransgenic progeny of event DP‐32138‐1	0/35 (0)	0/35 (0)	35/35 (100)
Nontransgenic progeny of event E6611.22.8.2	0/13 (0)	0/13 (0)	13/13 (100)
Nontransgenic progeny of event E6499.75.6.3	0/31 (0)	0/31 (0)	31/31 (100)

Self‐pollinated seeds segregate for DsRed2(Alt1) gene. Ms45, maize fertility restorer allele; zm‐aa1, α‐amylase gene; DsRed2(Alt1), red fluorescent gene; 35SEN, cauliflower mosaic virus 35S enhancer; Bt1, Brittle‐1 transit peptide; pMs45, Ms45 gene promoter; pLTP2, lipid transfer protein‐2 gene promoter; pMs5126, Ms*5126 gene promoter; pPG47, polygalacturonase gene promoter.