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Frequency of transgene editing using RTDS technology in Arabidopsis protoplasts. Oligonucleotides of three different lengths (nb) each possessing the C→T edit in codon H66 (H66Y; CAC→TAC) required to convert blue fluorescent protein (BFP) to green fluorescent protein (GFP) were delivered into Arabidopsis protoplasts by the PEG method. Percentage of GFP fluorescing cells was measure 72 h after delivery by cytometry. Error bars are mean ± SEM (n = 3).
