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. 2016 Oct 10;16:782. doi: 10.1186/s12885-016-2808-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Different effects of ΔNp63α on cell adhesion/proliferation in BT-20 and BT-549 cells. BT-20 and BT-549 cells were transiently transfected with pcDNA3-ΔNp63α and pcDNA3-GFP plasmids and analyzed by xCELLigence 24 h after transfection. Cell index was significantly decreased in BT-20 cells expressing ΔNp63α (p < 0.01, t-test) and MDA-MB-468-ΔNp63α cells treated with tetracycline (p < 0.01, t-test) but not in BT-549 cells expressing ΔNp63α (p = 0.15, t-test) after 48 h. Expression of ΔNp63α 24 h after transfection / tetracycline treatment was confirmed by western blot