Fig. 5.

Validation of selected ncRNAs by northern blot. Four newly discovered ncRNAs (ncRNA34, ncRNA83, ncRNA192, ncRNA200) were randomly selected for northern blot validation. For each selected ncRNA, the normalized coverage plots from RNA-seq data of aligned reads is shown on the top with the genomic context identified from the Mtb H37Rv (NC_000962.3) genome and the position of the ncRNA is indicated by coloured arrows. The coverage data is indicated with the following colours: no stress -black; H₂O₂ - red; MMC -blue; DETA/NO -green. RNA samples for the northern blots are from the correspondent samples. Control RNA is from non-stressed cells. For each northern blot, the loading control (5S rRNA) and decade marker are shown