Table 3.

Primers used in this study

Primer	Primer sequence (5′–3′)
P1	ACAACCACACACATCCACGTGATGCGACGATCCGGTAACTACTCCCCTT (PmlI)
P2	TTAGTTTCGGGTTCCCACGTGCTAGGCGAAAGGCTGGAACAGGCAA (PmlI)
P3	ACAACCACACACATCCACGTGATGTCCGCCCGAGGTCTCAACAAAA (PmlI)
P4	TTAGTTTCGGGTTCCCACGTGCTAGTAGGTGTGGCCACCGAATCGT (PmlI)
P5	AGATAGAGTCGACAAAGGCCTGCTAGCTTATCGATACGCGTGCATG (StuI)
P6	TGTACACCGAGAAACAGGCCTCATCTCACTTGCGTATGTATGGAAA (StuI)
P7	ACAACCACACACATCCACGTGATGCTACAAGCAGCTATTGG (PmlI)
P8	TTAGTTTCGGGTTCCCACGTGCTATGACCGTATGCAAATATT (PmlI)
P9	ACAACCACACACATCCACGTGATGACGACGTCTTACAGCGA (PmlI)
P10	TTAGTTTCGGGTTCCCACGTGCTACTTGATCCACCGCCGAA (PmlI)
P11	ACAACCACACACATCCACGTGATGACCACCTATTCGGCTCC (PmlI)
P12	TTAGTTTCGGGTTCCCACGTGCTACTTGAACCCCTTCTCGA (PmlI)
P13	ACAACCACACACATCCACGTGATGCGACTCACTCTGCCCCG (PmlI)
P14	TTAGTTTCGGGTTCCCACGTGCTACTCGACAGAAGAGACCT (PmlI)
P15	ACAACCACACACATCCACGTGATGGACTACATCATTTCGGC (PmlI)
P16	TTAGTTTCGGGTTCCCACGTGCTAATGGGTCCAGGGACCGA (PmlI)
P17	ACAACCACACACATCCACGTGATGATCCACCAGGCCTCCAC (PmlI)
P18	TTAGTTTCGGGTTCCCACGTGCTACTTGCTGTTCTTCAGAG (PmlI)
P19	CGAGGCAGCAGATCCACTAGTAGCACCGCCGCCGCAAGGAATGG (SpeI)
P20	GCGGCCGCATAGGCCACTAGTCTGTCAAACATGAGAATTCGG (SpeI)
P21	CTCTCAAGGGCATCGGTCGACAGCACCGCCGCCGCAAGGAATGG (SalI)
P22	CGCATAAGGGAGAGCGTCGACCTGTCAAACATGAGAATTCGG (SalI)
P23	CGGCATCCGCTTACAGAC
P24	GGAGGCATCAGTGACCAAA
P25	CATTAGGAAGCAGCCCAGTA
P26	GAGATCGTCAAGGGTTTG
P27	CATAAGTGCGGCGACGAT
P28	CTACTACTGGGCTGCTTCCTA

Restriction sites are underlined