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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: Chem Res Toxicol. 2016 Mar 11;29(3):323–332. doi: 10.1021/acs.chemrestox.5b00485

Table 2.

Sites of HNE Modification on Endogenous CDK2 Identified by LC-MS/MSa

peptide residue modified XCorr observed m/z charge mass error (ppm) peptide start–stop observed spectra
LLDVIH*TENK His71 3.58 670.3919 2 4.92 66–75 3
APEILLGC*K Cys177 2.87 1101.6543 1 3.99 170–178 5
a

RKO cells were transfected with CDK2-His and treated with HNE. Following isolation of His-tagged proteins and separation by SDS-PAGE, bands corresponding to CDK2-His were excised, subjected to in-gel digestion, and analyzed by tandem mass spectrometry. Modified residues are indicated by an asterisk. Data shown in the table represent the adducted peptides with the lowest mass error.