Table 1. SAXS-derived parameters for NLRP1(227–990).
| Data-collection Parameters | ||
| Instrument | SEC-SAXS at B21 Diamond Light source | |
| SEC column | Superdex 200 (GE Healthcare) | |
| q range (Å-1) | 0.0022–0.42 | |
| Temperature (°C) | 25°C | |
| Sample Concentration (before SEC) | 2mg/mL | 6mg/mL |
| Structural parameters | ||
| I(0) (cm-1/absorbance) [from P(r)] | 0.0018±0.0001 | 0.0067±0.0002 |
| Rg (Å) [from P(r)] | 34.6±0.4 | 35.0±0.1 |
| I(0) (cm-1/absorbance) (from Guinier) | 0.0018±0.0001 | 0.0066±0.0002 |
| Rg (Å) (from Guinier) | 34.1±0.4 | 34.8±0.2 |
| Dmax (Å) | 114 | 115 |
| Porod Volume estimate (Å3) | 139208 | 127250 |
| χ2 a | 0.96 | 1.02 |
| Normalised Spatial Discrepancy | 0.57±0.03 | 0.61±0.03 |
| SAXS derived Molecular Mass | ||
| From Porod Volume (VPorod/1.6) (Da) | 87000 | 79000 |
| SAXSMoW (Da)b | 101000 | 98000 |
| From BSA as standard | 99000 | 91000 |
| Molecular mass from sequence (kDa) | 89.5 | |
| Software employed | ||
| Primary data reduction | SCATTER | |
| Data processing | ATSAS | |
| Ab initio analysis | DAMMIF | |
| Validation and averaging | DAMAVER | |
| Computation of model intensities | CRYSOL | |
| Three-dimensional graphic representation | PyMOL | |
avalue derived from best DAMMIF model