Table 1. SID-MRM analysis of TNF-α and/or β-escin effect in HUVEC.

For SID-MRM tests proteins were selected on the basis of iTRAQ indications. Cells obtained from six different donors were used. Significant alternations according to Benjamini-Hochberg corrected p-values determined with t-test on respective regression coefficients are indicated by asterisks (***—p value of 0.0001 to 0.001; **—0.001 to 0.01 and *—0.01 to 0.05; ns–non significant).

iTRAQ indication	Protein name	Change upon TNF-α treatment	Change upon β-escin treatment	Change upon β-escin treatment of TNF-α induced cells
affected by TNF-α	Intercellular adhesion molecule 1 (ICAM-1 / ICAM1)	↑ ***	ns	ns
	Plasminogen activator inhibitor 1 (PAI-1 / SERPINE1)	↑ ***	ns	ns
	von Willebrand factor (vWF / VFW)	↓ ***	ns	ns
	Superoxide dismutase [Mn], mitochondrial (SOD2)	↑ ***	ns	↓ *
	Low affinity cationic amino acid transporter 2 (CAT-2 / SLC7A2)	↑ ***	ns	↓ *
	Tumor necrosis factor alpha-induced protein 2 (TNFAIP2)	↑ ***	ns	↓ **
	DnaJ homolog subfamily A member 1 (DNAJA1)	↑ **	ns	↓ **
	EH domain-containing protein 1 (EHD1)	↑ *	ns	↓ *
	COUP transcription factor 1 & 2 (COUP-TF1, COUP-TF2 / NR2F1, NR2F2)	↓ **	↓ *	ns
	Thrombospondin-1 (THBS1)	ns	ns	ns
affected by TNF-α and β-escin	Vascular cell adhesion protein 1 (VCAM-1 / VCAM1)	↑ ***	ns	↓ **
	E-selectin (SELE)	↑ ***	ns	ns
	Endothelial protein C receptor (EPCR / PROCR)	ns	↓ *	↓ **
	Multimerin-1 (MMRN1)	ns	ns	↓ *
	Hedgehog-interacting protein (HHIP)	ns	ns	ns
affected by β-escin	Cathepsin B (CTSB)	ns	↓ **	↓ ***
	Cathepsin Z (CTSZ)	ns	↓ **	↓ ***
	Palmitoyl-protein thioesterase 1 (PPT-1 / PPT1)	ns	↓ *	↓ ***
	CD63 antigen (LAMP-3 / CD63)	ns	↑ **	↑ ***
	Amyloid beta A4 protein (APP)	ns	↑ *	↑ **
	Integral membrane protein 2B (ITM2B)	ns	↑ **	↑ **
	Matrix metalloproteinase-14 (MMP-14 / MMP14)	ns	↑ ***	↑ **
	Podocalyxin (PODXL)	ns	↑ *	↑ **