Fig 1. Overview of experimental study design.

(a) Schematic of scanning ultrasound (SUS) setup. (b-f) Treatment and experimental scheme. A cohort of wild-type mice was treated with (b) a single SUS treatment, and (d) electrophysiological recordings were performed 2 hours or 24 hours later to investigate the acute affects of SUS on neuronal excitability. A second cohort of mice was treated with (c) six SUS treatments once per week for six weeks and allowed to age for one week or three months before (d) electrophysiology, (e) neuronal morphology and (f) dendritic spine density were investigated to determine how SUS treatment affects neuronal excitability and synaptic connectivity.