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. 2016 Oct 11;11(10):e0164278. doi: 10.1371/journal.pone.0164278

Fig 3. CA1 neuronal excitability is not impaired by multiple SUS treatments even after long time periods.

Fig 3

(a) Schematic of the experimental design of multiple treatments. (b) Representative traces of APs generated by injection of current steps (-60, 0, threshold and 320 pA). (c, d) Input-output relationships from sham- and SUS-treated neurons recorded (c) 1 week (1 w, p = 0.39, SUS 182.5 ± 18.2 a.u., sham 155.7 ± 22.7 a.u.) or (d) 3 months (3 m, p = 0.62, SUS 204.8 ± 12 a.u., sham 193.4 ± 18.78 a.u.) after the end of treatment. Quantification of (e) AP amplitude (1 w, p = 0.88, SUS 95.1 ± 1.3 mV, sham 95.6 ± 1.3 mV; 3 m, p = 0.67, SUS 101.4 ± 0.68 mV, sham 100.1 ± 1.3 mV), (f) rise-time (1 w, p = 0.13, SUS 142.9 ± 4.5 μs, sham 132.1 ± 3.6 μs; 3 m, p = 0.23, SUS 130.2 ± 2.52 μs, sham 137.2 ± 4.43 μs), (g) half-width (1 w, p<0.0001, SUS 0.89 ± 0.02 ms, sham 0.79 ± 0.02 ms; 3 m, p = 0.96, SUS 0.83 ± 0.008 ms, sham 0.84 ± 0.02 ms), (h) AP threshold (1 w, p = 0.63, SUS -46.2 ± 0.99 mV, sham -45.4 ± 1.58 mV; 3 m, p = 0.93, SUS -49.7 ± 0.75 mV, sham -49.4 ± 0.95 mV), (i) AHP (1 w, p = 0.34, SUS 5.8 ± 0.69 mV, sham 5.1 ± 0.69 mV; 3 m, p = 0.0011, SUS 5.9 ± 0.62 mV, sham 3.8 ± 0.94 mV), (j) sag (1 w, p = 0.10, SUS 1.5 ± 0.16 mV, sham 1.2 ± 0.13 mV; 3 m, p>0.99, SUS 1.3 ± 0.11 mV, sham 1.3 ± 0.11 mV), (k) AP amplitude adaptation (1 w, p = 0.21, SUS 76.3 ± 2.32%, sham 73.8 ± 2.33%; 3 m, p = 0.0081, SUS 76.6 ± 0.94%, sham 80.0 ± 1.47%), (l) AP frequency adaptation (1 w, p = 0.71, SUS 64.1 ± 4.23%, sham 60.0 ± 4.37%; 3 m, p = 0.08, SUS 66.3 ± 2.90%, sham 58.5 ± 3.52%), (m) input resistance (1 w, p = 0.28, SUS 117.5 ± 6.97 MΩ, sham 112.5 ± 10.69 MΩ; 3 m, p = 0.58, SUS 110.7 ± 6.53 MΩ, sham 105.8 ± 8.07 MΩ), (n) time constant (1 w, p = 0.08, SUS 10.5 ± 0.55 ms, sham 13.5 ± 1.16 ms; 3 m, p = 0.31, SUS 12.3 ± 0.62 ms, sham 12.4 ± 0.77 ms) and (o) capacitance (1 w, p = 0.0016, SUS 94.0 ± 6.88 pF, sham 122.2 ± 5.52 pF; 3 m, p = 0.49, SUS 131.3 ± 9.40 pF, sham 118.9 ± 2.82 pF). Data are presented as mean ± SEM and as individual data points. **p<0.01 and ***p<0.001. Sample size: one week time-point—SUS n = 19 neurons and sham n = 15 neurons. 3 month time-point—SUS n = 27 neurons and sham n = 24 neurons.