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. 2016 Oct 11;11(10):e0164278. doi: 10.1371/journal.pone.0164278

Fig 4. Multiple SUS treatments prevent dendrite loss of CA1 pyramidal neurons.

Fig 4

(a) Fluorescent z-projection of a recovered fluorescently labeled neuron used for morphometric analysis. (b) Representative 2D projection of 3D reconstructions of recorded neurons from SUS- and sham-treated mice. (c-g) Quantification of (c) the number of apical dendrite branch nodes (SUS versus sham 1 w, p = 0.88, SUS 31.6 ± 2.71 nodes, sham 30.8 ± 2.43 nodes; SUS versus sham 3 m, p = 0.0119, SUS 31.4 ± 1.52 nodes, sham 25.1 ± 1.73 nodes; 1 w versus 3 m sham, p = 0.028, 1 w 30.8 ± 2.43 nodes, 3 m 25.1 ± 1.73 nodes; 1 w versus 3 m SUS, p = 0.69, 1 w 31.6 ± 2.71 nodes, 3 m 31.4 ± 1.52 nodes), (d) total apical dendrite length (SUS versus sham 1 w, p = 0.66, SUS 3312 ± 223.7 μm, sham 3465 ± 308.2 μm; SUS versus sham 3 m, p = 0.0017, SUS 3210 ± 118.1 μm, sham 2592 ± 143.6 μm; 1 w versus 3 mo sham, p = 0.0346, 1 w 3465 ± 308.2 μm, 3 m 2592 ± 143.6 μm; 1 w versus 3 m SUS, p = 0.76, 1 w 3312 ± 223.7 μm, 3 m 3210 ± 118.1 μm), (e) 3D dendritic tree volume (SUS versus sham 1 w, p = 0.35, SUS 5.00x106 ± 0.39x106 μm3, sham 6.4x106 ± 1.15x106 μm3; SUS versus sham 3 m, p = 0.0038, SUS 6.0x106 ± 0.42x106 μm3, sham 4.0x106 ± 0.42x106 μm3; 1 w versus 3 m sham, p = 0.11, 1 w sham 6.4x106 ± 1.15x106 μm3, 3 m 4.0x106 ± 0.42x106 μm3; 1 w versus 3 m SUS, p = 0.17, 1 w 5.00x106 ± 0.39x106 μm3, 3 m 6.0x106 ± 0.42x106 μm3) (f) the number of basal dendrite branch nodes (1 w, p = 0.77, SUS 12.3 ± 1.53 nodes, sham 11.5 ± 1.84 nodes; 3 m, p = 0.81, 15.8 ± 1.37 nodes, sham 15.2 ± 1.65 nodes), (g) total basal dendrite length (1 w, p = 0.42, SUS 1335 ± 202.8 μm, sham 1584 ± 256.9 μm; 3 m, p = 0.33, SUS 1834 ± 167.5 μm, sham 1596 ± 176.6 μm). (h-k) Quantification of the dendritic branch order (h, SUS versus sham 1 w, p = 0.063, F (1, 832) = 0.23; i, SUS versus sham 3 m, p = 0.0001, F (1, 1280) = 17.93; j, 1 w versus 3 m SUS, p = 0.41, F (1, 1248) = 0.68; k, 1 w versus 3 m sham, p = 0.0001, F (1, 864) = 15.26). Data are presented as mean ± SEM and as individual data points. *p<0.05, **p<0.01 and ***p<0.001. Sample size: one week time-point—SUS n = 16 neurons and sham n = 12 neurons. 3 month time-point—SUS n = 25 neurons and sham n = 18 neurons.