Table 4.

Humanness of RK35 Constructs.

Variable Domain	Perfect 9mers	HSC	SeqID	SeqSim	T20
RK35_VH_murine	51	87	78	89	80.43
RK35-VH_1.0	74	90	89	91	89.18
RK35-VH_1.2	94	95	95	97	95.35
RK35-VH_1.3	81	93	93	95	93.66
RK35-VH_1.4	75	92	91	91	90.69
RK35-VH_1.5	74	91	90	92	89.66
RK35_VL_murine	10	79	70	86	73.79
RK35-VL_1.0	54	89	86	92	89.44
RK35-VL_1.2	67	93	91	94	90.89
RK35-VL_1.3	71	96	95	97	95.28
RK35-VL_1.4	79	96	96	97	96.31
RK35-VL_1.5	72	96	96	98	95.93

Perfect 9mers are the number of 9-mers in the sequence that can be found in a human germline.²³ HSC is the Human String Content score.²³ SeqID and SeqSim are the sequence identity or sequence similarity to the DP47 or DPK9 germlines. T20 is the humanness of monoclonal antibody variable region sequences as compared to the human antibody repitoire.⁵⁹