Figure 1.
Binding properties of anti-human ETB specific monoclonal antibodies. To evaluate maximal binding (A) and apparent affinities (B, C), CHO and UACC-257 cells expressing hETB were incubated for 24 h at 4°C with increasing concentrations of ETB directed antibodies. Binding of anti-ETB antibodies were revealed using R-PE-labeled anti-mouse antibody and quantified by flow cytometry. Each antibody's maximal binding (Bmax) and apparent affinity (1/apparent Kd) were determined using GraphPad Prism software.