Figure 9.

Rendomab-B4 inhibits melanoma cell migration induced by endothelin. UACC-257 (150 nM) seeded in culture inserts were treated for 2 h with or without 150 nM of rendomab-B4 or control isotype antibody before the addition of 10 nM ET-1. After 20 h of incubation, cells were stained with crystal violet and counted from 3 randomly selected images per insert. (A) Images of UACC-257 cells on the lower side of the insert membranes. (B) Quantification of the number of cells present on the lower side of the insert membranes. Values are the means ± SEM of 3 independent experiments.