Figure 1.

Humanization and affinity reduction of antibody CA645. The heavy (A) and light (B) chain sequences of antibody CA645 are aligned with human germline acceptor framework sequences VH3 1-3 3-23/JH4 and Vκ1 2-1-(1) L5/Jκ4. Rabbit residues are in red, human residues are in black and CDRs are in blue (J-region CDR residues are shown but acceptor V-region CDRs are not). The grafted VH (gH) and VL (gL) sequences are shown below their corresponding human acceptor germline frameworks. Framework sequence differences between the rabbit and human framework sequences are shown with asterisks. Rabbit framework residues retained in the humanized grafts are highlighted in bold.