Figure 7.
Determination of the equilibrium dissociation constant (KD) of the selected anti-EGFR Nbs using surface plasmon resonance. SPR sensorgrams monitoring real-time association and dissociation of purified Nbs VEGFR1-5 (at the concentrations indicated in the color code box) to eEGFR-Fc immobilized onto a CM5 sensor chip. The increase in resonance units (RU) was recorded along time (in seconds) and dissociation of the Nbs was evaluated after injection of buffer. Sensorgrams from 2 independent injections are shown. The curve was fitted by non-linear least squares regression using the 1:1 Langmuir binding model (theoretical sensorgrams in black).