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. 2016 Jul 29;8(7):1286–1301. doi: 10.1080/19420862.2016.1216742

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 9. — Competition of EGF and the selected Nbs for binding to eEGFR-Fc determined by flow cytometry analysis of E. coli bacteria. Flow cytometry analysis of induced E. coli EcM1 bacteria displaying the indicated Nb (VEGFR1-6) and incubated with 50 nM of biotinylated BSA (black) or eEGFR-Fc in the absence (red) or presence (blue) of 25 µM EGF. Overlay of histograms showing the fluorescence intensity of bacteria after staining with secondary Streptavidin-PE.