Figure 2.

Assessing by NMR and SEC the long-term storage stability of mAb2 at 40°C in selected formulations. The 1D ¹H NMR spectral overlays (amide and aromatic region) for 4 different formulations of 300 mg/ml mAb2 in 10 mM citrate-phosphate buffer are shown, as a function of time: at pH 6 in the absence of additives (A); in the presence of 200 mM Arg·Glu (B); at pH 7 in the presence of 200 mM Arg·Glu (C); and in the presence of 200 mM Arg·HCl (D). Correspondent panels (E)-(H) show for the same 4 formulations the time-dependence of relative fractions of aromatic (F^AR) and amide (F^NH) signals remaining in the spectra vs time, reporting on soluble protein loss. Independently, the fraction of monomeric protein F^mono was assessed using SEC and plotted. (Color version of this figure is available online)