Skip to main content
. 2016 Apr 13;7(21):29937–29943. doi: 10.18632/oncotarget.8729

Figure 2. HSC frequency and function in young and aged WT and S6K1−/− mice.

Figure 2

A. LSK frequency (percentage of total BM), B. frequency (percentage of total BM) of LT-HSC, HPC-1, HPC-2 and MPP in young WT and S6K1−/− mice (n = 9-11, mixed gender). C. Percentage of chimerism (CD45.2+ cells) in peripheral blood (PB) from irradiated recipients transplanted with young WT or S6K1−/− LT-HSCs (donor cells female, recipients mixed gender, n = 6-9). The effect of genotype on chimerism was non-significant (F = 3.037, P = 0.088), although the effect of time was (F = 5.696, P = 0.002). D. LSK frequency and E. frequency of LT-HSC, HPC-1, HPC-2 and MPP in BM of aged WT and S6K1−/− mice (n = 7-15, mixed gender). F. Percentage of chimerism (CD45.2+ cells) in PB from irradiated recipients transplanted with aged WT or S6K1−/− LT-HSCs (donor cells female, recipients mixed gender, n = 6). A highly significant genotype effect was detected (F = 8.452, P = 0.006), but the effect of time was non-significant (F = 0.494, P = 0.688). (* P < 0.05, ** P < 0.01, *** P < 0.001). Values are mean±SEM, with closed bars indicating WT mice and open bars indicating S6K1−/− mice.