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. 2016 Apr 19;7(21):29989–30002. doi: 10.18632/oncotarget.8802

Figure 4. Inhibition of ATG5 gene expression alleviated PrP (106-126)-induced cytotoxicity.

Figure 4

A. ATG5 small interfering RNA (siATG5) or negative control siRNA (NC) transfected SK-N-SH neuronal cells were incubated with 100 μM PrP (106-126) for 6h. Western blot for LC3-II and p62 proteins was analyzed from SK-N-SH cells. Beta-actin was used as the loading control. B. Bar graph indicating the volume of ATG5 expression levels. C. Cell viability was measured by annexin V assay. siATG5 or NC transfected SK-N-SH neuron cells were incubated with 100 μM PrP (106-126) for 24h. D. Bar graph indicating the average number of annexin V negative cells. * p < 0.05, ** p < 0.01, *** p < 0.001; significant differences between each treatment group. PrP, Prion peptide (106-126); adj.volume, adjustment of volume (band volume minus background volume).