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. 2015 May 24;7(21):30068–30083. doi: 10.18632/oncotarget.4027

Figure 4. BAL and lung tissue inflammation are increased in aged mice.

Figure 4

A. The lungs of young and aged mice were lavaged with 4 × 0.5 ml aliquots of sterile PBS and total and differential cell counts were determined. B. BAL cytokine secretion of MCP1 (CCL2), KC (CXCL1), MIP-1α (CCL3), IL-6 and TNF-α was determined using a magnetic bead-based multiplex assay. For this assay, BAL fluid was concentrated (10x) by ultrafiltration in centrifugal filter devices. C. Lung tissue mRNA expression of MCP1 (CCL2), KC (CXCL1), MIP-1α (CCL3), IL-6 and TNF-α was measured by qPCR. Data were combined from 2 independent experiments with n = 8 and are given as mean values ± SD; one-way ANOVA following Bonferroni post test with *p < 0.05, **p < 0.01, ***p < 0.001.