Table 6. Suggested quality parameters for variant calls in other implementation studies for NGS.
Source | Total reads per sample | Coverage for variant calls | Allele frequency | Variant calls in for and rev strands |
---|---|---|---|---|
[1] | − | >500x | >5% | − |
[11] | − | >50x | − | − |
[44] | − | >80x | >20% | + |
[45] | >300,000 | >500x | >10% | − |
[46] | >100,000 | >500x | − | − |
“–” indicates no statement about the specific parameter in this study.