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. 2016 Mar 23;7(21):30084–30093. doi: 10.18632/oncotarget.8310

Table 6. Suggested quality parameters for variant calls in other implementation studies for NGS.

Source Total reads per sample Coverage for variant calls Allele frequency Variant calls in for and rev strands
[1] >500x >5%
[11] >50x
[44] >80x >20% +
[45] >300,000 >500x >10%
[46] >100,000 >500x

“–” indicates no statement about the specific parameter in this study.