Table 7. Minimum quality parameters suggested for mutation profiling in molecular diagnostic.

Quality parameter	Explanation
5% variant allele frequency of the variant call	Probability of validation with other sequencing technologies
Forward and reverse strands show the variant	Variants detected only in one strand are high likely artifacts
>500x coverage at the variant position	Enough sequencing reads for proper mutation calling
PHRED-scaled quality of 100	Low probability of incorrect variant call